An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast

Julle H. Cocker; Simonetta Piatti; Corrado Santocanale; Kim Nasmyth; John F.X. Diffley

doi:10.1038/379180a0

An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast

Julle H. Cocker, Simonetta Piatti, Corrado Santocanale, Kim Nasmyth, John F.X. Diffley

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

311 Citations (Scopus)

Abstract

Origins of DNA replication in Saccharomyces cerevisiae are bound by two protein complexes during the cell cycle. Post-replicative complexes closely resemble those generated in vitro by purified origin recognition complex (ORC), which is essential for DNA replication in vivo. Pre-replicative complexes (pre-RCs) are characterized by an extended region of nuclease protection overlapping the ORC footprint. We show here that the Cdc6 protein (Cdc6p), which is necessary for origin firing in vivo, is essential for the establishment and maintenance of pre-RCs, suggesting that it is a component of these complexes. Without Cdc6p, G1 origins closely resemble post-replicative origins, providing evidence that ORC is also a component of pre-RCs. These results suggest that pre-RCs play an essential role in initiating DNA replication and support a two-step mechanism for the assembly of functional initiation complexes.

Original language	English
Pages (from-to)	180-182
Number of pages	3
Journal	Nature
Volume	379
Issue number	6561
DOIs	https://doi.org/10.1038/379180a0
Publication status	Published - 11 Jan 1996
Externally published	Yes

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title = "An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast",

abstract = "Origins of DNA replication in Saccharomyces cerevisiae are bound by two protein complexes during the cell cycle. Post-replicative complexes closely resemble those generated in vitro by purified origin recognition complex (ORC), which is essential for DNA replication in vivo. Pre-replicative complexes (pre-RCs) are characterized by an extended region of nuclease protection overlapping the ORC footprint. We show here that the Cdc6 protein (Cdc6p), which is necessary for origin firing in vivo, is essential for the establishment and maintenance of pre-RCs, suggesting that it is a component of these complexes. Without Cdc6p, G1 origins closely resemble post-replicative origins, providing evidence that ORC is also a component of pre-RCs. These results suggest that pre-RCs play an essential role in initiating DNA replication and support a two-step mechanism for the assembly of functional initiation complexes.",

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T1 - An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast

AU - Cocker, Julle H.

AU - Piatti, Simonetta

AU - Santocanale, Corrado

AU - Nasmyth, Kim

AU - Diffley, John F.X.

PY - 1996/1/11

Y1 - 1996/1/11

N2 - Origins of DNA replication in Saccharomyces cerevisiae are bound by two protein complexes during the cell cycle. Post-replicative complexes closely resemble those generated in vitro by purified origin recognition complex (ORC), which is essential for DNA replication in vivo. Pre-replicative complexes (pre-RCs) are characterized by an extended region of nuclease protection overlapping the ORC footprint. We show here that the Cdc6 protein (Cdc6p), which is necessary for origin firing in vivo, is essential for the establishment and maintenance of pre-RCs, suggesting that it is a component of these complexes. Without Cdc6p, G1 origins closely resemble post-replicative origins, providing evidence that ORC is also a component of pre-RCs. These results suggest that pre-RCs play an essential role in initiating DNA replication and support a two-step mechanism for the assembly of functional initiation complexes.

AB - Origins of DNA replication in Saccharomyces cerevisiae are bound by two protein complexes during the cell cycle. Post-replicative complexes closely resemble those generated in vitro by purified origin recognition complex (ORC), which is essential for DNA replication in vivo. Pre-replicative complexes (pre-RCs) are characterized by an extended region of nuclease protection overlapping the ORC footprint. We show here that the Cdc6 protein (Cdc6p), which is necessary for origin firing in vivo, is essential for the establishment and maintenance of pre-RCs, suggesting that it is a component of these complexes. Without Cdc6p, G1 origins closely resemble post-replicative origins, providing evidence that ORC is also a component of pre-RCs. These results suggest that pre-RCs play an essential role in initiating DNA replication and support a two-step mechanism for the assembly of functional initiation complexes.

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An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast

Abstract

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