Abstract
The literature is conflicted on the influence of ammonium on the kinetics and microbial ecology of methanotrophy. In this study, methanotrophic cultures were enriched, under ammonium concentrations ranging from 0 to 200 mM, from an inoculum comprising leachate and top-cover soil from a landfill. Specific CH4 biodegradation rates were highest (7.8 x 10(-4) + - 6.0 x 10(-5)gCH(4)g(x)(-1)h(-1)) in cultures enriched at 4 mM NH4+, which were mainly dominated by type II methanotrophs belonging to Methylocystis spp. Lower specific CH4 oxidation rates (average values of 1.8-3.6 x 10(-4)gCH(4)g(x)(-1)h(-1)) were achieved by cultures enriched at higher NH4+ concentrations (20 and 80 mM), and had higher affinity for CH4 compared to 4 mM enrichments. These lower affinities were attributed to lower diversity dominated by type I methanotrophs, of the Methylosarcina, Methylobacter and Methylomicrobium genera, encountered with increasing concentrations of NH4+. The study indicates that CH4 oxidation biotechnologies applied at low NH4+ concentrations can support efficient abatement of CH4 and high diversity of methanotrophic consortia, whilst enriching type II methanotrophs. (C) 2019 Elsevier Ltd. All rights reserved.
| Original language | English (Ireland) |
|---|---|
| Pages (from-to) | 345-353 |
| Number of pages | 9 |
| Journal | Waste Management |
| Volume | 89 |
| DOIs | |
| Publication status | Published - 1 Apr 2019 |
Keywords
- Ammonium
- Methane biodegradation kinetics
- Methanotrophs
- Methanotrophy
- Microbial diversity
- Nitrification
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Lopez, JC., Porca, E., Collins, G., Clifford, E., Quijano, G., Munoz, R.
