Alternatively Spliced mRNAs for Human Endothelin-2 and Their Tissue Distribution

G. Oreilly; D. S. Charnockjones; J. J. Morrison; I. T. Cameron; A. P. Davenport; S. K. Smith

doi:10.1006/bbrc.1993.1701

Alternatively Spliced mRNAs for Human Endothelin-2 and Their Tissue Distribution

G. Oreilly, D. S. Charnockjones, J. J. Morrison, I. T. Cameron, A. P. Davenport, S. K. Smith

University of Cambridge

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

31 Citations (Scopus)

Abstract

The cDNA for Endothelin-2 (ET-2) has been previously cloned and characterised; however, ET-2 remains the least studied of the endothelin isopeptides and little is known of its function and location. In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven alternatively spliced mRNA variants encoding ET-2, with a specific pattern of distribution in various human tissues. Computer alignment and analysis of the DNA sequences demonstrated alternative splicing of five exons of 52, 169, 123, 99 and 174 base pairs, in the carboxy terminal region of the mRNA encoding preproET-2. This region contains sites for the post-transcriptional processing of preproET-2 into mature ET-2, therefore we postulate that post-transcriptional processing may be disrupted or altered in these variants.

Original language	English
Pages (from-to)	834-840
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	193
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.1701
Publication status	Published - 30 Jun 1993
Externally published	Yes

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title = "Alternatively Spliced mRNAs for Human Endothelin-2 and Their Tissue Distribution",

abstract = "The cDNA for Endothelin-2 (ET-2) has been previously cloned and characterised; however, ET-2 remains the least studied of the endothelin isopeptides and little is known of its function and location. In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven alternatively spliced mRNA variants encoding ET-2, with a specific pattern of distribution in various human tissues. Computer alignment and analysis of the DNA sequences demonstrated alternative splicing of five exons of 52, 169, 123, 99 and 174 base pairs, in the carboxy terminal region of the mRNA encoding preproET-2. This region contains sites for the post-transcriptional processing of preproET-2 into mature ET-2, therefore we postulate that post-transcriptional processing may be disrupted or altered in these variants.",

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AU - Oreilly, G.

AU - Charnockjones, D. S.

AU - Morrison, J. J.

AU - Cameron, I. T.

AU - Davenport, A. P.

AU - Smith, S. K.

PY - 1993/6/30

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N2 - The cDNA for Endothelin-2 (ET-2) has been previously cloned and characterised; however, ET-2 remains the least studied of the endothelin isopeptides and little is known of its function and location. In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven alternatively spliced mRNA variants encoding ET-2, with a specific pattern of distribution in various human tissues. Computer alignment and analysis of the DNA sequences demonstrated alternative splicing of five exons of 52, 169, 123, 99 and 174 base pairs, in the carboxy terminal region of the mRNA encoding preproET-2. This region contains sites for the post-transcriptional processing of preproET-2 into mature ET-2, therefore we postulate that post-transcriptional processing may be disrupted or altered in these variants.

AB - The cDNA for Endothelin-2 (ET-2) has been previously cloned and characterised; however, ET-2 remains the least studied of the endothelin isopeptides and little is known of its function and location. In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven alternatively spliced mRNA variants encoding ET-2, with a specific pattern of distribution in various human tissues. Computer alignment and analysis of the DNA sequences demonstrated alternative splicing of five exons of 52, 169, 123, 99 and 174 base pairs, in the carboxy terminal region of the mRNA encoding preproET-2. This region contains sites for the post-transcriptional processing of preproET-2 into mature ET-2, therefore we postulate that post-transcriptional processing may be disrupted or altered in these variants.

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U2 - 10.1006/bbrc.1993.1701

DO - 10.1006/bbrc.1993.1701

M3 - Article

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Alternatively Spliced mRNAs for Human Endothelin-2 and Their Tissue Distribution

Abstract

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