TY - JOUR
T1 - Agar Plate-Based Method for the Selection of Antibiotic-Resistant Bacterial Strains
AU - Zeden, Merve S.
AU - Gröndling, Angelika
N1 - Publisher Copyright:
© 2023 Cold Spring Harbor Laboratory Press. All rights reserved.
PY - 2023/11
Y1 - 2023/11
N2 - Identifying the molecular mechanisms underlying antibiotic resistance is important, as it can reveal key information on the mode of action of a drug and provide insights for the development of novel or improved antimicrobials. Here, we describe an agar-based method for the selection of bacterial strains with increased antibiotic resistance, and how the increase in resistance can be confirmed by a spotplating assay. As a specific example, we describe the selection of Staphylococcus aureus strains with increased resistance to oxacillin; however, the protocol can be easily adapted and used with other bacteria and antibiotics.
AB - Identifying the molecular mechanisms underlying antibiotic resistance is important, as it can reveal key information on the mode of action of a drug and provide insights for the development of novel or improved antimicrobials. Here, we describe an agar-based method for the selection of bacterial strains with increased antibiotic resistance, and how the increase in resistance can be confirmed by a spotplating assay. As a specific example, we describe the selection of Staphylococcus aureus strains with increased resistance to oxacillin; however, the protocol can be easily adapted and used with other bacteria and antibiotics.
UR - https://www.scopus.com/pages/publications/85178946014
U2 - 10.1101/pdb.prot107897
DO - 10.1101/pdb.prot107897
M3 - Article
SN - 1940-3402
VL - 2023
SP - 816
EP - 821
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 11
ER -