A rapid, convenient enzyme linked immunosorbent assay for total immunoglobulin a in serum

M. C. Booth; J. P. Gosling; P. F.F. Fottrell; C. F. McCarthy

doi:10.1007/BF02937257

A rapid, convenient enzyme linked immunosorbent assay for total immunoglobulin a in serum

M. C. Booth, J. P. Gosling, P. F.F. Fottrell, C. F. McCarthy

Molecular Biosciences

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

3 Citations (Scopus)

Abstract

A Specific, sensitive and convenient Elisa for total IgA in serum, carried out on microtitre plates and based on commercially available reagents, was developed and extensively validated. The cross-reactivity of both IgG and IgM was much less than 0.1% and other serum proteins were undetectable. The detection limit was 0.18 ng/well or 90 micrograms/ml of serum, allowing for pre-dilution. Sera from normal (1.4-4.1 mg/ml), coeliacs (1.6-13.52 mg/ml) and IgA deficient individuals (0.05-0.4 mg/ ml) were analysed.

Original language	English
Pages (from-to)	146-152
Number of pages	7
Journal	Irish Journal of Medical Science
Volume	154
Issue number	4
DOIs	https://doi.org/10.1007/BF02937257
Publication status	Published - Apr 1985

Access to Document

10.1007/BF02937257

Cite this

@article{a0babce488a5440695a2404242fdb05e,

title = "A rapid, convenient enzyme linked immunosorbent assay for total immunoglobulin a in serum",

abstract = "A Specific, sensitive and convenient Elisa for total IgA in serum, carried out on microtitre plates and based on commercially available reagents, was developed and extensively validated. The cross-reactivity of both IgG and IgM was much less than 0.1% and other serum proteins were undetectable. The detection limit was 0.18 ng/well or 90 micrograms/ml of serum, allowing for pre-dilution. Sera from normal (1.4-4.1 mg/ml), coeliacs (1.6-13.52 mg/ml) and IgA deficient individuals (0.05-0.4 mg/ ml) were analysed.",

author = "Booth, {M. C.} and Gosling, {J. P.} and Fottrell, {P. F.F.} and McCarthy, {C. F.}",

year = "1985",

month = apr,

doi = "10.1007/BF02937257",

language = "English",

volume = "154",

pages = "146--152",

journal = "Irish Journal of Medical Science",

issn = "0021-1265",

number = "4",

}

TY - JOUR

T1 - A rapid, convenient enzyme linked immunosorbent assay for total immunoglobulin a in serum

AU - Booth, M. C.

AU - Gosling, J. P.

AU - Fottrell, P. F.F.

AU - McCarthy, C. F.

PY - 1985/4

Y1 - 1985/4

N2 - A Specific, sensitive and convenient Elisa for total IgA in serum, carried out on microtitre plates and based on commercially available reagents, was developed and extensively validated. The cross-reactivity of both IgG and IgM was much less than 0.1% and other serum proteins were undetectable. The detection limit was 0.18 ng/well or 90 micrograms/ml of serum, allowing for pre-dilution. Sera from normal (1.4-4.1 mg/ml), coeliacs (1.6-13.52 mg/ml) and IgA deficient individuals (0.05-0.4 mg/ ml) were analysed.

AB - A Specific, sensitive and convenient Elisa for total IgA in serum, carried out on microtitre plates and based on commercially available reagents, was developed and extensively validated. The cross-reactivity of both IgG and IgM was much less than 0.1% and other serum proteins were undetectable. The detection limit was 0.18 ng/well or 90 micrograms/ml of serum, allowing for pre-dilution. Sera from normal (1.4-4.1 mg/ml), coeliacs (1.6-13.52 mg/ml) and IgA deficient individuals (0.05-0.4 mg/ ml) were analysed.

UR - http://www.scopus.com/inward/record.url?scp=0021945917&partnerID=8YFLogxK

U2 - 10.1007/BF02937257

DO - 10.1007/BF02937257

M3 - Article

C2 - 3891671

AN - SCOPUS:0021945917

SN - 0021-1265

VL - 154

SP - 146

EP - 152

JO - Irish Journal of Medical Science

JF - Irish Journal of Medical Science

IS - 4

ER -

A rapid, convenient enzyme linked immunosorbent assay for total immunoglobulin a in serum

Abstract

Access to Document

Other files and links

Fingerprint

Cite this