A modified rapid enzymatic microtiter plate assay for the quantification of intracellular γ-aminobutyric acid and succinate semialdehyde in bacterial cells

C. P. O'Byrne; C. Feehily; R. Ham; K. A.G. Karatzas

doi:10.1016/j.mimet.2010.10.017

A modified rapid enzymatic microtiter plate assay for the quantification of intracellular γ-aminobutyric acid and succinate semialdehyde in bacterial cells

C. P. O'Byrne, C. Feehily, R. Ham, K. A.G. Karatzas

Infectious Disease

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

19 Citations (Scopus)

Abstract

The GABase assay is widely used to rapidly and accurately quantify levels of extracellular γ-aminobutyric acid (GABA). Here we demonstrate a modification of this assay that enables quantification of intracellular GABA in bacterial cells. Cells are lysed by boiling and ethanolamine-O-sulphate, a GABA transaminase inhibitor is used to distinguish between GABA and succinate semialdehyde.

Original language	English
Pages (from-to)	137-139
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	84
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2010.10.017
Publication status	Published - Jan 2011

Keywords

Acid tolerance
GABA
GABase
Listeria monocytogenes
Succinate semialdehyde

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10.1016/j.mimet.2010.10.017

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abstract = "The GABase assay is widely used to rapidly and accurately quantify levels of extracellular γ-aminobutyric acid (GABA). Here we demonstrate a modification of this assay that enables quantification of intracellular GABA in bacterial cells. Cells are lysed by boiling and ethanolamine-O-sulphate, a GABA transaminase inhibitor is used to distinguish between GABA and succinate semialdehyde.",

keywords = "Acid tolerance, GABA, GABase, Listeria monocytogenes, Succinate semialdehyde",

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AU - Feehily, C.

AU - Ham, R.

AU - Karatzas, K. A.G.

PY - 2011/1

Y1 - 2011/1

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AB - The GABase assay is widely used to rapidly and accurately quantify levels of extracellular γ-aminobutyric acid (GABA). Here we demonstrate a modification of this assay that enables quantification of intracellular GABA in bacterial cells. Cells are lysed by boiling and ethanolamine-O-sulphate, a GABA transaminase inhibitor is used to distinguish between GABA and succinate semialdehyde.

KW - Acid tolerance

KW - GABA

KW - GABase

KW - Listeria monocytogenes

KW - Succinate semialdehyde

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DO - 10.1016/j.mimet.2010.10.017

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JF - Journal of Microbiological Methods

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A modified rapid enzymatic microtiter plate assay for the quantification of intracellular γ-aminobutyric acid and succinate semialdehyde in bacterial cells

Abstract

Keywords

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