Abstract
A three-electrode electrochemical biosensor has been developed using printed circuit boards for detecting bacterial contamination. This low-cost, small-size device consists of thin-film gold electrodes and is fabricated using photo-lithography paired with electro-deposition. Pathogen presence is deduced by detection of change in electrical impedance caused by binding of the pathogen to bio-receptors coated on the sensor surface. The biosensor has a total area of similar to 3.2 cm(2) and requires only 100 mu L of test sample for detection. The sensor geometry has been optimized using techniques from Design of Experiments, and the device can be operated using a small ac excitation potential of magnitude 5 mV. The sensor is tested on the common food-borne pathogen Salmonella typhimurium and is able detect bacterial concentrations of the order of 500 CFU mL within 6 min. In this paper, the design and fabrication of the biosensor is detailed along with the experiments that validate its performance.
| Original language | English (Ireland) |
|---|---|
| Pages (from-to) | 210-216 |
| Number of pages | 6 |
| Journal | Ieee Sensors Journal |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2011 |
Keywords
- Biomedical transducers
- design automation
- electrochemical processes
- impedance measurement
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Nandakumar, V;Bishop, D;Alonas, E;LaBelle, J;Joshi, L;Alford, TL