A fluorescently labeled, hyperbranched polymer synthesized from DE-ATRP for the detection of DNA hybridization

  • Ahmed Aied
  • , Barry Glynn
  • , Hongliang Cao
  • , Yu Zheng
  • , Hongyun Tai
  • , Abhay Pandit
  • , Wenxin Wang

Research output: Contribution to a Journal (Peer & Non Peer)Articlepeer-review

15 Citations (Scopus)

Abstract

The early detection of oligonucleotide biomarkers of disease, such as microRNAs, has been established as a fundamental factor in cancer diagnosis. As the levels of these small molecules (microRNAs) in blood have recently been found to be significantly affected in cancer patients, they offer a means of early stage detection of cancer. Towards the goal of creating a novel method of DNA hybridization detection, we report the detection of specific sequences of small oligonucleotides in a model experiment carried out in serum. The results shown here display the versatility of the DE-ATRP method in synthesizing a specific polymer structure capable of changing its physical properties in the presence of double stranded DNA. The polymer was labeled and used to detect single-stranded DNA in serum successfully.

Original languageEnglish
Pages (from-to)332-334
Number of pages3
JournalPolymer Chemistry
Volume3
Issue number2
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

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