Abstract
Three commonly used transfection techniques (electroporation, calcium phosphate precipitation and scrape loading) and a novel procedure combining the latter two methods were evaluated and conditions optimised for successful transfection of human HepG2 cells with plasmid DNA incorporating a mouse MHC Class I gene and a selectable marker (neomycin transferase gene) conferring resistance to G418. While transfection with linear DNA by scrape-loading gave satisfactory results, transfer of cloned circular DNA by electroporation, calcium phosphate precipitation or a combined use of scrape-loading and calcium phosphate gave best results for HepG2 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1059-1066 |
| Number of pages | 8 |
| Journal | Biochemistry and Molecular Biology International |
| Volume | 32 |
| Issue number | 6 |
| Publication status | Published - 1994 |
| Externally published | Yes |
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