[8] Interfering and Complicating Adsorption Effects in Bioaffinity Chromatography

This β-galactosidase system has played an important role in the development of the view that immobilized ligands with dissociation constants in the millimolar range are capable of promoting very strong specific adsorption, and this view in turn has led to the development of the concept of “progressively perpetuating effectiveness.” This concept is not consistent with much other evidence, or with the theoretical treatment summarized in the next section, and it is difficult to reconcile with kinetic theory in general. Again, the observations that largely gave rise to this concept are explainable in terms of nonbiospecific rather than biospecific adsorption.